Co-cultivation of vascular endothelial and smooth muscle cells using microcarrier techniques |
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Authors: | Peter F. Davies Catherine Kerr |
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Affiliation: | 1. Vascular Pathophysiology Laboratory, Department of Pathology, Brigham, USA;2. Women''s Hospital, Harvard Medical School, Boston, MA 02115, USA |
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Abstract: | A system is described which uses microcarrier culture techniques for the co-cultivation of different cell types without direct contact between cell populations. In co-cultivation, arterial endothelial cells induced proliferation in > 90% of quiescent homologous arterial smooth muscle cells in the absence of serum-derived growth factors. The microcarrier coculture system allows investigation of potent local humoral interactions between vascular cells in vitro. |
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