Somatic embryogenesis from immature zygotic embryos of oil palm |
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Authors: | J. B. Teixeira M. R. Söndahl E. G. Kirby |
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Affiliation: | (1) DNA Plant Technology Corp., 2611 Branch Pike, 08077 Cinnaminson, N.J., USA;(2) Department of Biological Sciences, Rutgers University, 07102 Newark, N.J., USA;(3) Present address: CENARGEN, EMBRAPA, P.O. Box 02372, Brasilia, DF, Brazil |
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Abstract: | Immature zygotic embryos at different developmental stages were used for callus induction and regeneration studies. Immature embryos excised from fruits 77, 91, 100, 114, 128, 140 and 193 days after pollination and mature embryos were cultured on modified Y3 medium containing 500 mgl–1 cysteine, 0.5% (w/v) PVP-40, 500 M 2,4-d and 0.3% (w/v) charcoal. Compact embryogenic tissue began differentiating directly from embryo explants after 2 weeks of culture. The percentage of embryos forming compact embryogenic tissue ranged from 28.6% for 91-day-old embryos to 0% for 140-day-old and older embryos. Friable embryogenic tissue was observed in callus cultures derived from 100-day-old embryos. Although both compact and friable embryogenic tissues were successfully isolated, normal embryo and plantlet development was observed only from friable embryogenic tissue.Abbreviations ABA abscisic acid - 2,4-d 2,4-dichlorophenoxyacetic acid - NAA naphthaleneacetic acid - PVP polyvinylpyrollidone |
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Keywords: | Elacis guineeses |
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