Selection of downy mildew resistant somaclones,from a susceptible B line of pearl millet |
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Authors: | Umesha S. Nagarathna K.C. Shetty Sudheer A. Shekar Shetty H. |
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Affiliation: | (1) Downy Mildew Research Laboratory, Department of Studies in Applied Botany, University of Mysore, Manasagangotri, Mysore, 570 006, India;(2) Present address: # 221, Shelley Rence, Cordova, TN 38018, USA;(3) Labland Biotechs Private Limited, KRS Main Road, Metagalli, Mysore, 570 016, India |
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Abstract: | Plants regenerated from seed-derived callus of a PNMS 6B line of pearl millet (Pennisetum glaucum (L.) R. Br.) were evaluated for their resistance induced by somaclonal variation for downy mildew disease caused by Sclerospora graminicola (Sacc.) Schroter. Among the 201 lines regenerated, only 3 lines consistently proved highly resistant (free from disease incidence) for up to 5 generations; whereas, 17 lines were resistant (disease incidence ranging from 1 to 9%). Resistance was confirmed by testing the plants under both laboratory and field conditions. The plants were evaluated for their agronomic traits. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | agronomic traits Pennisetum glaucum Sclerospora graminicola somaclonal variation tissue culture |
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