Nif plasmid from Lignobacter |
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Authors: | Mieczyslawa Derylo Maria Glowacka Anna Skorupska Zbigniew Lorkiewicz |
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Institution: | (1) Institute of Microbiology, University M. Curie-Sklodowska, Akademicka 19, PL-20-033 Lublin, Poland |
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Abstract: | Lignobacter strain K17 is able to degrade aromatic compounds and to fix atmospheric nitrogen. It was proved that capacity for nitrogen fixation by Lignobacter was plasmid mediated. Plasmid pUCS100 (17.5 Mdal) carrying nif genes was transferred from Lignobacter to Escherichia coli SK1592 and Salmonella typhimurium. The transposon Tn9 was translocated to pUCS100 to facilitate selection of Nif+ bacteria. E. coli SK1592 harboring the new plasmid (pUCS101) reduced acetylene under anaerobic conditions. Plasmids pUCS100 and pUCS101 were not stably maintained in E. coli and S. typhimurium.Abbreviations Mdal
megadalton
- CsCl-EtBr
caesium chloride ethidium bromide
- m.o.i.
multiplicity of infection |
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Keywords: | Lignobacter Salmonella typhimurium Escherichia coli Nif plasmid Tn9 Transduction Transformation |
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