酿酒酵母中SSK2基因与其他镉耐受相关基因之间的双基因缺失菌株构建

镉是一种严重的环境污染物,对人体具有致癌性,能蓄积在生物体内影响机体的生长、发育和生殖。有丝分裂原蛋白激酶(Mitogen-activated protein kinase,MAPK)在调节细胞存活、增殖和分化中是重要的信号分子,并能够被镉胁迫激活。酿酒酵母中2个MAPK信号传导途径,高渗透压甘油(High Osmolarity Glycerol,HOG)途径和细胞壁完整性(Cell Wall Integrity,CWI)途径都参与Cd2+胁迫下的细胞应答。为了进一步研究这两条途径在调控Cd2+胁迫方面的相互作用,以HOG途径的蛋白激酶SSK2基因为例,通过合成遗传阵列(Synthetic Genetic Array,SGA)方法,成功构建了SSK2基因与其他52个Cd2+耐受相关基因之间的双基因缺失菌株。为大规模研究Cd2+耐受基因之间在调控镉胁迫方面的遗传学相互作用奠定了基础,也为酿酒酵母的相关研究提供了一个新的遗传学手段。

Construction of Double-Gene Deletion Mutants between SSK2 & other Cadmium-Tolerant Genes in Wine-making Yeast

Cadmium is a serious environmental pollutant, it has carcinogenicity to human body, and it could accumulate in bions affecting their growth, development and reproduction. Mitogen activate protein (MAP) kinases are important signaling molecules regulating cell survival, proliferation and differentiation, and they can be activated by cadmium stress. Two MAP kinase signaling pathways, the cell wall integrity (CWI) pathway and high osmolarity and glycerol (HOG) pathway, are involved in the response to cadmium stress in Saccharomyces cerevisiae. In order to study their genetic interactions in regulating cadmium stress, SSK2 was used as an example and constructed its double gene deletion mutants with other 52 cadmium tolerant genes in the study, using the synthetic genetic array (SGA) method. The study provides a basis for understanding the genetic interaction between cadmium tolerant genes and a new genetic means for related studies in wine making yeast.