粘质沙雷氏菌几丁质酶基因ChiA克隆及生物信息学分析

通过生物信息学技术对Chi A基因序列进行分析预测,了解Chi A的基因结构及蛋白质性质。从自有菌株(粘质沙雷氏菌Serratia mareescens S68)中克隆到几丁质酶基因Chi A,利用相关软件对Chi A基因序列进行分析预测。Chi A基因全长1 714 bp,开放阅读框编码563个氨基酸,推测其编码的蛋白质分子量为60 983.8Da,等电点为6.35,是一种稳定的亲水性蛋白质。预测Chi A可能存在信号肽,切割位点在第23~24位氨基酸之间,1~23位氨基酸为其跨膜结构,其余肽链位于细胞外。Chi A主要存在3种二级结构元件,在二级、三级结构中都有体现。该Chi A是一种水溶性蛋白质,结构稳定且可以分泌到胞外。

Cloning & Bioinformatics Analysis of Chitinase Gene ChiA from Serratia marcescens

In order to learn the structure of chitinase gene (ChiA) and the protein properties of ChiA, the sequence of ChiA was analyzed and predicted by bioinformatics techniques. ChiA was cloned from Serratia mareescens strain 41003, its sequence was analyzed and predicted by bioinformatics software. The results showed that the length of ChiA was 1 714 bp having an open reading frame encoding 563 amino acids inferring their encoded protein molecular weight at 60 983.8 Da with isoelectric point at 6.35, it was a stable hydrophilic protein. It was predicted that it might exist a signal peptide in ChiA with its cutting site between 23rd to 24th amino acid. 1st to 23rd amino acid might be the transmembrane construction and the rest peptide chains located outside the cell.ChiA mainly existed three kinds of secondary structure elements, which embodied in both secondary structure and tertiary structure. Therefore,ChiA was a hydrosoluble protein with stable structure and could be secreted outside the cell.