Genetic diversity and population genetic structure of saltmarsh Spartina alterniflora from four coastal Louisiana basins |
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Authors: | Herry S Utomo Ida Wenefrida Michael D Materne Stephen A Harrison |
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Institution: | 1. Rice Research Station, Louisiana Agricultural Experiment Station, Louisiana State University, Agricultural Center, 1373 Caffey Road, Rayne, LA 70578, USA;2. School of Plant, Soil, and Environmental Sciences, Louisiana State University Agricultural Center, 104 Sturgis Hall, Baton Rouge, LA 70803, USA |
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Abstract: | Seventy-two Spartina alterniflora accessions originating from four coastal Louisiana basins (18 accessions per basin) were used to evaluate the genetic structure of this native perennial low-intertidal plant species. The objective of this study was to determine the population genetic structure and diversity of S. alterniflora accessions originating from these four basins using amplified fragment length polymorphism (AFLP) markers. A total of 250 unambiguous and highly repeatable AFLP markers, 186 of which (74.4%) were polymorphic, were obtained using four primer combinations. Overall, pairwise similarity estimates between accessions ranged from 0.70 to 0.93 (average = 0.80) with only a small portion of alleles (0.54–1.08%) unique to each basin. The average Hs (genetic diversity within coastal basins) was 0.20 with an Hs values of 0.19, 0.20, 0.20, and 0.21 for Mermentau, Terrebonne, Calcasieu, and Barataria-Breton basin, respectively. AMOVA analysis showed no genetic structure among basins, with the majority of genetic variation, 96.6%, residing within the basins. There was no indication of isolation by distance. Our results suggest that maintaining high levels of genetic diversity can be accomplished through the use of an adequate number of S. alterniflora samples collected within any large basin. Choosing parental lines from several Louisiana coastal basins for breeding purposes may not significantly increase genetic variability among the progeny lines. |
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Keywords: | Spartina alterniflora Smooth cordgrass Genetic diversity DNA marker Population structure Coastal erosion control Habitat restoration |
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