Production of inulotriose from inulin by inulin-degrading enzyme from Streptomyces rochei E87

A. YOKOTA, O. YAMAUCHI AND F. TOMITA. 1995. A strain of streptomycctes producing an extracellular inulin-degrading enzyme was isolated from a soil sample. This strain was identified as Streptomyces rochei E87. The inulin-degrading enzyme was found to degrade inulin into inulotriose as the main end product. This enzyme was induced by inulin. Under favourable culture conditions, the enzyme activity in the culture supernatant fluid reached 1.0 U ml^-1after incubation for 3 d. Using this enzyme, inulotriose was produced in a weight yield of over 70% from an initial 10–50 gl^-1of inulin.