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A study of morphology,cytology and sterility in interspecific hybrids and amphidiploids of Nicotiana knightiana X N. umbratica
Authors:T Gangadevi  P N Rao  B H Rao  K V Satyanarayana
Institution:(1) Department of Botany, Andhra University, Visakhapatnam, India;(2) Division of Genetics and Cytogenetics, Central Tobacco Research Institute, Rajahmundry, India
Abstract:Summary Interspecific hybrids and amphidiploids of Nicotiana knightiana Goodspeed (n= 12)x N. umbratica Burbidge (n = 23) resembled either parent in some characters and were intermediate in other characters. The F1 hybrids (2n = 35) showed mostly univalents during meiosis, while the amphidiploids (2n = 70) formed bivalents almost regularly. The former were completely sterile and the latter fully male fertile but predominantly female sterile. This female sterility was due to disintegration of the embryo sacs leading to collapsed ovules. The few fertile ovules, however, showed normal development of embryo sac and embryo. The occurrence of fertile and sterile ovules was believed to be due to segregation of the genes governing sterility.
Keywords:Nicotiana knightiana  N  umbratica  Interspecific hybrids  Cytology  Female sterility
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