Strategies for the identification and purification of ligands for orphan biomolecules |
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Authors: | Nice Edouard Catimel Bruno Lackmann Martin Stacker Steven Runting Andrew Wilks Andrew Nicola Nicos Burgess Antony |
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Institution: | (1) Ludwig Institute for Cancer Research, 3050 Melbourne, VIC, Australia;(2) The Walter and Eliza Hall Institute of Medical Research, 3050 Melbourne, VIC, Australia;(3) The Cooperative Research Center for Cellular Growth Factors, 3050 Melbourne, VIC, Australia |
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Abstract: | Summary The isolation of related genes with evolutionary conserved motifs by the application of polymerase chain reaction-based molecular
biology techniques, or from database searching strategies, has facilitated the identification of new members of protein families.
Many of these protein molecules will be involved in protein-protein interactions (e.g. growth factors, receptors, adhesion
molecules), since such interactions are intrinsic to virtually every cellular process. However, the precise biological function
and specific binding partners of these novel proteins are frequently unknown, hence they are known as ‘orphan’ molecules.
Complementary technologies are required for the identification of the specific ligands or receptors for these and other orphan
proteins (e.g., antibodies raised against crude biological extracts or whole cells). We describe herein several alternative
strategies for the identification, purification and characterisation of orphan peptide and protein molecules, specifically
the synergistic use of micropreparative HPLC and biosensor techniques.
These authors made equivalent contributions. |
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Keywords: | Micropreparative HPLC Protein-protein interactions Biosensors Surface plasmon resonance Cytosensor |
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