Electrophoretic Pattern of Larval Esterases in Field and Laboratory-selected Strains of the Tobacco Cutworm,Spodoptera litura (Fabricius) |
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Institution: | 1. Faculty of Land and Food Systems, University of British Columbia, Vancouver, V6T 1Z4 BC, Canada;2. Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine, Pharmacy Research Center, Tehran University of Medical Sciences, 14155/6451 Tehran, Iran |
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Abstract: | This research was performed to find out and characterize the specific esterase isozymes related to OP and pyrethroid resistance in the tobacco cutworm Spodoptera litura (Fabricius). Two laboratory strains (DSR5 and CSR4) of S. litura, selected with deltamethrin and chlorpyrifos-methyl, had higher larval esterase activities than Hamancollected (HC) strain. Ten esterase isozymes were separated in the wild HC strain on 6.5% nondenaturing polyacrylamide gel electrophoresis (pH 8.3), but only 5 of them were detected in the two strains selected with insecticides. The isozymes were designated from E1 to E10 according to their mobility to cathode. E4 was stained more strongly in both laboratory-selected strains than in HC strain. The frequency of E2 in DSR5 strain was higher than in HC strain. E2 and E4 were proved to be arylesterase and carboxyesterase, respectively, according to enzyme inhibition tests. Thus decreased number and increased intensity of esterase bands in DSR5 and CSR4 strain may be associated with insecticide resistance, resulting from selections successively with insecticides for several generations. |
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