E. coli ribosomal proteins are cross reactive with antibody prepared against Chlamydomonas reinhardi chloroplast ribosomal subunit |
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Authors: | Richard Schneeman and Stefan Surzycki |
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Affiliation: | (1) Department of Biology, Indiana University, 47 405 Bloomington, Indiana, USA;(2) Present address: Sloan-Kettering Institute for Cancer Research (Walker Laboratory), 145 Boston Post Road, 10 580 Rye, New York, USA |
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Abstract: | Summary Antisera prepared against purified Chlamydomonas reinhardi small chloroplast ribosomal subunit, judged homogenous by sucrose gradient velocity sedimentation and RNA gel electrophoresis was immunologically cross reactive with E. coli ribosomal proteins. The results of three different experimental approaches, namely Ouchterlony double diffusion, sucrose gradient velocity sedimentation and two dimensional crossed immunoelectrophoresis indicate that both E. coli ribosomal subunits and the chloroplast large ribosomal subunit contain proteins which show antigenic similarity to the chloroplast small ribosomal subunit proteins. However, cytoplasmic ribosomal subunits did not contain proteins which were cross reactive with immune antisera. |
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