Caleosins: Ca2+-binding proteins associated with lipid bodies |
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Authors: | Næsted Henrik Frandsen Gitte I Jauh Guang-Yuh Hernandez-Pinzon Immaculada Nielsen Henrik B Murphy Denis J Rogers John C Mundy John |
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Institution: | (1) Institute of Molecular Biology, Oester Farimagsgade 2A, 1353 Copenhagen K, Denmark;(2) Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA;(3) John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK |
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Abstract: | We have previously identified a rice gene encoding a 27 kDa protein with a single Ca2+-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1–5). Northern hybridization demonstrated that AtClo2–4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain. |
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Keywords: | Arabidopsis thaliana calcium-binding protein caleosin EF-hand gene expression lipid bodies vesicles |
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