Light-stimulated GTP binding to a membrane protein in rod outer segments. |
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| Authors: | R L Somers H Shichi |
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| Affiliation: | Laboratory of Vision Research, National Eye Institute, National Institutes of Health, U.S. Department of Health, Education, and Welfare, Bethesda, MD 20205 USA |
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| Abstract: | Bovine as well as frog rod outer segments contain a membrane-bound protein which binds the GTP analog GppNp in the light (Kd=0.3μM). The amount of GppNp bound is 2.5–3.5 mmole per mol rhodopsin. The binding protein (M.Wt. ? 54,000) can be extracted from rod membranes with detergent and purified on an Agarose column. The chromatographic profile indicates that the binding protein is distinct from rhodopsin, GTPase or cyclic nucleotide phosphodiesterase. |
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