基于TMT技术的盐胁迫下罗布麻的蛋白质组学分析

罗布麻(Apocynum venetum L.),是我国宝贵的野生植物种质资源,具有重要的药用价值、生态价值及经济价值。罗布麻虽属于耐盐性较强的植物,但随着生境遭到破坏及土壤盐渍化程度加重,罗布麻的野生种群数量逐渐缩减,探究罗布麻的耐盐性机制,对罗布麻野生种质资源的保护和利用具有重要意义。本研究采用TMT技术对盐胁迫下罗布麻进行定量蛋白质组学研究,对不同时间点差异蛋白进行韦恩发现,11个共有差异蛋白主要富集在蛋白质-FAD连接、转运蛋白活性的负调控、离子跨膜转运蛋白活性的负调节、阴离子跨膜转运的负调控、阴离子通道活性的负调节和噻唑代谢过程等通路。差异蛋白网络互作分析发现,不同胁迫时间的差异蛋白网络互作所得到的蛋白主要以核糖体蛋白为主,其中60S核糖体蛋白L3-2(TRINITY_DN13265_c0_g1_i1_9)在胁迫12 h和24 h的差异蛋白中均为核心蛋白,表明核糖体蛋白在罗布麻响应盐胁迫过程中发挥着重要作用。本研究筛选出罗布麻响应盐胁迫的关键蛋白,拓展了罗布麻盐胁迫应答相关的分子资源,并为罗布麻天然抗逆种质的发掘与利用提供理论依据。

TMT-based Technologyproteomic Analysis of Apocynum venetum under Salt Stress

Apocynum venetum L. is a precious wild plant germplasm resource in China, which has important medicinal value, ecological value and economic value. Although A. venetum is a plant with strong salt tolerance, the wild population has gradually decreased with the destruction of habitat and the increase of soil salinization. To explore the mechanism of salt tolerance of A. venetum is of great significance for the protection and utilization of wild germplasm resources. In this study, TMT technology was used to conduct quantitative proteomic research on A. venetum under salt stress, and different proteins at different time points were found by Venn analysis. The 11 common differential proteins were mainly concentrated in protein-FAD linkage, negative regulation of transporter activity, negative regulation of ion transmembrane transporter activity, negative regulation of anion transmembrane transport, negative regulation of anion channel activity, and thiazole metabolism. The interaction analysis of differential protein networks showed that ribosomal proteins were the main proteins obtained by the interaction of differential protein networks at different stress times, among which the ribosomal protein L2-2 (TRINITY_DN13265_c0_g1_i1_9) was the core protein in the differential proteins at 12 h and 24 h under stress. The results indicated that ribosomal proteins played an important role in the response of A. venetum to salt stress. In this study, the key proteins of A. venetum in response to salt stress were screened, the molecular resources related to A. venetum salt stress response were expanded, and the theoretical basis was provided for the discovery and utilization of natural resistance germplasm of A. venetum.