Preparation of Porcine Neurophysin Proteins by High Performance Liquid Chromatography |
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Authors: | W Richter P Schwandt |
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Institution: | Medical Department II, Klinikum Grosshadern, University of Munich, Marchioninistr. 15, 8000 Munich 70, W. Germany |
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Abstract: | Abstract: The crude neurophysin containing extract from posterior lobes of porcine pituitaries was roughly purified by gel chromatography. 15 mg of the lyophilized neurophysin complex were completely separated by HPLC yielding in neurophysin I1 (3.6 mg), I2 (4.0 mg), II (4.6 mg) and III (1.9 mg). All of the neurophysins were homogenous by PAGE and SDS-electrophoresis, isoelectrofocussing, amino-acid composition and N- and C-terminal amino acid analysis. In conclusion, HPLC is a reliable and quick method for the preparation of pure neurophysins. |
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Keywords: | Neurophysin proteins Porcine pituitary Preparation HPLC |
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