Determination of the activity and concentration of immobilized and soluble enzymes by microcalorimetry |
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| Authors: | NN Rehak J Everse NO Kaplan RL Berger |
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| Institution: | 1. Laboratory of Technical Development, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014 USA;1. Department of Chemistry, University of California, La Jolla, California 92037 USA |
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| Abstract: | The relationship between activity and concentration of both soluble and immobilized lactate dehydrogenase and uricase was determined using the rate of heat production in a batch-type microcalorimeter. The results demonstrate the feasibility of using microcalorimetry for the determination of enzyme levels in biological fluids and for the determination of the activity of particle-bound enzymes. |
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