Toxicity analysis of encapsulation solutions and polymers in the cultivation of insect cells |
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Authors: | N. A. Smith M. F. A. Goosen G. A. King P. Faulkner A. J. Daugulis |
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Affiliation: | (1) Department of Chemical Engineering, Queen's University, K7L 3N6 Kingston, Ontario, Canada;(2) Department of Microbiology and Immunology, Queen's University, K7L 3N6 Kingston, Ontario, Canada |
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Abstract: | Summary Microencapsulation of insect cells, hosts for baculovirus expression systems, requires that the encapsulation reagents and membrane-forming materials be non-toxic to the cells. Various encapsulation polymers (poly-l-lysine, chitosan and alginate) and solutions (KCl, CaCl2, CHES and sodium citrate) were tested for their toxicity toSpodopterafrugiperda cells. The effects of varying polymer molecular weight and concentration on cell viability were also investigated. |
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