Time course study on accumulation of cell wall-bound phenolics and activities of defense enzymes in tomato roots in relation to <Emphasis Type="Italic">Fusarium</Emphasis> wilt |
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Authors: | Sudhamoy Mandal Adinpunya Mitra Nirupama Mallick |
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Institution: | (1) Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India;(2) Central Horticultural Experiment Station (ICAR), Aiginia, Bhubaneswar, 751019, India |
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Abstract: | Major cell wall-bound phenolic compounds were detected and identified in roots of tomato at different stages of growth. Alkaline
hydrolysis of the cell wall material of the root tissues yielded ferulic acid as the major bulk of the phenolic compounds.
Other phenolic compounds identified were 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin and 4-coumaric
acid. All the six phenolic acids were higher in very early stage of plant growth. Ferulic acid, 4-hydroxybenzoic acid and
4-coumaric acid exhibited a decreasing trend up to 60 days and then the content of these phenolic acids increased somewhat
steadily towards the later stage of growth. Total phenolics, phenylalanine ammonia-lyase (PAL) activity and peroxidase (POD)
activity were in tandem match with the occurrence pattern of the phenolic acids. Ferulic acid showed highest antifungal activity
against tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici. The results of this study may be interpreted to seek an explanation for high susceptibility of tomato plants at flowering
stage to Fusarium wilt. It may also be concluded that greater amounts of ferulic acid in combination with other phenolics and higher level
of PAL and POD activities after 60 days of growth may have a role in imparting resistance against Fusarium wilt at a late stage of plant growth. |
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Keywords: | Antimicrobial activity Ferulic acid Fusarium oxysporum f sp lycopersici Peroxidase Phenylalanine ammonia-lyase Solanum lycopersicum Total phenolics |
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