Morphogenetic behavior of simian virus 40-transformed human mammary epithelial stem cell lines on collagen gels |
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Authors: | Philip S Rudland Gillian E Ollerhead Angela M Platt-Higgins |
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Institution: | (1) Cancer and Polio Research Fund Laboratories, Department of Biochemistry, University of Liverpool, P.O. Box 147, L69 3BX Liverpool, United Kingdom |
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Abstract: | Summary Transformation of primary cultures of human breast cells with simian virus 40 and clonal selection has yielded single-cell-cloned,
epithelial cell lines, as well as myoepithelial-related cell lines. When grown on floating collagen gels, the epithelial cell
lines give rise to branching rays of cells, thick fingerlike protrusions, saclike structures, and degenerating areas. The
myoepithelial-related cell lines give rise only to the branching rays. Epidermal growth factor stimulates the production of
the thick protrusions, whereas cholera toxin stimulates the production of the degenerating areas. Immunocytochemical staining
of these cultures using reagents directed against the cell surface-extracellular matrix or the cellular cytoskeleton confirms
the epithelial and myoepithelial nature of the cells, and demonstrates that the degenerating areas are undergoing squamous
metaplasia. The fingerlike protrusions consist of cords of cells composed of inner, epithelial and outer, myoepithelial-related
cells sometimes surrounding a central lumen reminiscent of ducts. The saclike structures resemble alveoli. Ultrastructural
analysis confirms the identification of the basic cell types and also identifies indeterminate cells possessing features of
both epithelial and myoepithelial cells. It is suggested that the epithelial cell lines represent human mammary stem cells
that can undergo processes of morphogenesis and differentiation in vitro to form many of the three-dimensional structures
found within the breast.
This work was supported by the North West Cancer Research Fund and the Cancer and Polio Research Fund. |
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Keywords: | breast stem cell lines collagen gels |
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