Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells. |
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| Authors: | Michiko Sasabe Kana Naito Hiroko Suenaga Takako Ikeda Kazuhiro Toyoda Yoshishige Inagaki Tomonori Shiraishi Yuki Ichinose |
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| Institution: | Laboratory of Plant Pathology & Genetic Engineering, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan. msasabe@bio.nagoya-u.ac.jp |
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| Abstract: | The inhibition of elicitor-induced plant defense responses by the protein kinase inhibitors K252a and staurosporine indicates that defense responses require protein phosphorylation. We isolated a cDNA clone encoding Nicotiana tabacum lectin-like receptor protein kinase 1 (NtlecRK1), an elicitor-responsive gene; in tobacco bright yellow (BY-2) cells by a differential display method. NtlecRK forms a gene family with at least three members in tobacco. All three NtlecRK genes potentially encode the N-terminal legume lectin domain, transmembrane domain and C-terminal Ser/Thr-type protein kinase domain. Green fluorescent protein (GFP) fusion showed that the NtlecRK1 protein was located on the plasma membrane. In addition, NtlecRK1 and 3 were responsive to INF1 elicitin and the bacterial elicitor harpin. These results indicate that NtlecRKs are membrane-located protein kinases that are induced during defense responses in BY-2 cells. |
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