Determination of the enantiomeric composition of mexiletine and its four hydroxylated metabolites in urine by enantioselective capillary gas chromatography |
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Authors: | Beate Knoche,Bä rbel Gehrcke,Wilfried A. Kö nig,Irving W. Wainer |
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Abstract: | The enantiomers of mexiletine and four of its hydroxylated metabolites were directly separated by capillary gas chromatography using a heptakis(6-O-tert-butyl-dimethylsilyl-2,3-di-O-methyl)-β-cyclodextrin column. The method was applied to the analysis of urine samples from cancer patients who were treated with racemic mexiletine as part of a study of the use of mexiletine in the relief of neuropathic pain. Samples analyzed before and after deconjugation of the urine with β-glucuronidase/arylsulfatase showed a high stereoselectivity in the formation and conjugation of these compounds. © 1996 Wiley-Liss, Inc. |
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Keywords: | gas chromatography modified β -cyclodextrin column chiral separation assay mexiletine hydroxylated metabolites urine conjugation/deconjugation |
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