The culture of human skin fibroblasts on microcarriers in the presence of serum substitute: Ultroser G |
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| Institution: | 1. School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;2. Lab of Plant Cell Biology, Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China;3. Shandong University of Science and Technology, Qingdao 266590, China;4. Chinese Research Academy of Environmental Sciences, Beijing 100021, China;5. School of Science and Engineering, Saitama University, Saitama 338-8570, Japan;1. School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Melbourne 3010, Victoria, Australia;2. Research Division, Peter MacCallum Cancer Centre, St Andrews Place, East Melbourne, Victoria, Australia;3. Department of Anatomical Pathology, The Alfred Hospital, Victoria 3181, Australia |
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| Abstract: | The effects of the substitution of serum by Ultroser G on human skin fibroblasts cultured on microcarriers were analysed. Cultures could not be established on microcarriers in the presence of Ultroser G. However, microcarrier cultures started in the presence of 10% foetal calf serum, and transferred to 2% Ultroser G after 7 days resulted in high cell densities. |
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