Epidermal-dermal and fibronectin cell-interactions during fish scale regeneration: immunofluorescence and TEM studies |
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| Affiliation: | 1. Equipe Formations Squelettiques, CNRS UA 041137, Laboratoire d''Anatomie Comparée France;2. Equipe de Biologie du Développement, Université Paris 7, 2 place Jussieu, F-75251 Paris Cedex 05, France;1. School of Biology and Food Engineering, Changshu Institute of Technology, Jiangsu, PR China;2. School of Tea and Food Science & Technology, Anhui Agricultural University, Anhui, PR China;3. Department of Biotechnology, Faculty of Bioscience Engineering, Center for Microbial Ecology and Technology (CMET), Ghent University, Ghent, Belgium;1. Radiology Informatics Lab, Department of Radiology, Mayo Clinic, Rochester, Minnesota;2. Radiology Department, Khon Kaen University, Khon Kaen, Thailand;1. School of Food Science and Engineering, South China University of Technology, 381 Wushan Road, Guangzhou, 510640, Guangdong, China;2. Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality, Institute for Environmental and Research, Jinan University, Guangzhou, 510632, China;3. Food & Nutritional Sciences Program, School of Life Sciences, Chinese University of Hong Kong, Hong Kong, China;1. Food Safety and Technology Research Center, Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong;2. School of Public Health (Shenzhen), Sun Yat-sen University, Guangzhou, Guangdong, China |
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| Abstract: | Fibronectin (FN) immunolocalization and ultrastructural observations of epidermal-dermal cell interactions during skin regeneration of the cichlid fish Hemichromis bimaculatus after scale removal were carried out for a healing period of 48 h. On control samples after scale removal, FN was localized on the surface of a thin cellular sheet, the scale-pocket lining (SPL), separating the dermis from the scale-associated cells. A layer of extracellular matrix, constituting a basement membrane, was observed on the SPL surface. Study of wound healing showed that in less than 1 h epithelial cells spread rapidly over the undamaged SPL surface on which FN was still present after the scale was removed. It appeared that this FN can be damaged or lost if the area is exposed to the exterior water for more than one hour. When epithelial continuity was re-established, an epithelial-SPL space was formed and was progressively filled by an electron-dense extracellular matrix. No basement membrane was defined until both tissues were separated by dermal cells about 30 h after the wound was inflicted. FN is detected in the epithelial-SPL interface from 12 h to 30 h after scale removal. Therefore, in normal skin, specific localization of FN in the interface between the scale-associated cells and the outer surface of the SPL cells can be implicated in the attachment between the scale and the SPL surface favoring a flexibility of scale movement during swimming. In addition, FN could play an important role as a major extracellular matrix protein during wound-healing after scale removal. |
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