Differential regulation of keratin 8 and 18 messenger RNAs in differentiating F9 cells |
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| Institution: | 1. Eye Center, the 2nd Affiliated Hospital, Medical College of Zhejiang University, No.1 Xihu Boulevard, Hangzhou 310009, China;2. Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China;3. MOE Key Laboratory of Macromolecule Synthesis and Functionalization of Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang Province, China;4. Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China;1. State Key Laboratory of Natural Medicines and Jiang Su Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China;2. Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China;1. Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA;2. UC Berkeley/UC San Francisco Graduate Group in Bioengineering, Berkeley, CA 94720, USA;3. Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA;4. Chan Zuckerberg Biohub, San Francisco, CA 94158, USA;1. Centre for Cell Biology of Chronic Disease, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Queensland, 4072 Australia;2. School of Physics, Sydney, NSW 2052, Australia;3. EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia |
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| Abstract: | F9 embryonal carcinoma cells (F9EC) can be induced to differentiate in vitro into epithelial cells expressing keratin 8 (K8) and keratin 18 (K18). cDNAs corresponding to K8 and K18 mRNAs were cloned and used to study the change in the abundance of these mRNAs during differentiation of F9 cells into parietal endoderm-like cells by treatment with retinoic acid (RA) or with RA and dibutyryl cAMP (Bt2cAMP). Using an RNase protection assay, it was determined that K8 mRNA was induced slightly before K18 mRNA and that it accumulated to a greater extent than K18 mRNA. Furthermore, differentiation in presence of Bt2cAMP plus RA resulted in an earlier induction of the two mRNAs and a higher level of expression of K8 mRNA. These results indicate that K8 and K18 mRNAs are regulated differently in F9 cells. |
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