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Rapid assessment of the effect of ciprofloxacin on chromosomal DNA from Escherichia coli using an in situ DNA fragmentation assay |
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| Authors: | María Tamayo Rebeca Santiso Jaime Gosalvez Germán Bou José Luis Fernández |
| Affiliation: | (1) INIBIC-Complejo Hospitalario Universitario A Coru?aUnidad de Gen?tica, As Xubias 84, 15006- A Coru?a, Spain;(2) Laboratorio de Gen?tica Molecular y Radiobiolog?aCentro Oncol?gico de Galicia, Avda. de Montserrat s/n, 15009-A Coru?a, Spain;(3) Unidad de Gen?tica, Facultad de Biolog?a, Universidad Aut?noma de Madrid, 28049 Madrid, Spain;(4) INIBIC-Complejo Hospitalario Universitario A Coru?aServicio de Microbiolog?a, As Xubias, 84, 15006-A Coru?a, Spain |
| Abstract: | BackgroundFluoroquinolones are extensively used antibiotics that induce DNA double-strand breaks (DSBs) by trapping DNA gyrase and topoisomerase IV on DNA. This effect is usually evaluated using biochemical or molecular procedures, but these are not effective at the single-cell level. We assessed ciprofloxacin (CIP)-induced chromosomal DNA breakage in single-cell Escherichia coli by direct visualization of the DNA fragments that diffused from the nucleoid obtained after bacterial lysis in an agarose microgel on a slide. |
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