Osteogenic and adipogenic differentiation potential of an immortalized fibroblast-like cell line derived from porcine peripheral blood |
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Authors: | Xiuqing Wang Daphne Moutsoglou |
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Institution: | (1) Department of Biology and Microbiology, Center for Infectious Disease Research and Vaccinology, South Dakota State University, NPB 252D, Box 2140D, Brookings, SD 57007, USA |
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Abstract: | Primary fibroblast-like cells isolated from the peripheral blood of a healthy pig were immortalized by transduction of cells
with a replication-defective retrovirus vector expressing the E6/E7 proteins of human papillomavirus type 16 (pLXSN-16E6E7).
The immortalized cells grow rapidly in cell culture and exhibit a distinct cell surface phenotype that was positive for CD90,
CD44, collagen I, and vimentin and negative for CD14 and MHC II. Additionally, these immortalized blood derived-fibroblast-like
cells had the potential to differentiate into osteoblasts and adipocytes in vitro as evidenced by the deposition of calcium,
increased alkaline phosphatase activity, upregulated osteogenic and adipogenic marker gene expression, and accumulation of
fat droplets in cells when osteogenic (dexamethasone, ascorbic acid, and β-glycerophosphate) or adipogenic supplements (dexamethasone,
3-isobutyl-1-methylxanthine, indomethacin, and insulin) were added to the culture. Overall, the results suggest that the immortalized
blood-derived fibroblast-like cells exhibit some of the features of mesenchymal precursor cells, which may have implications
in tissue repair and remodeling process. |
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