卵形鲳鲹胚胎及早期仔鱼耗氧量的研究

运用SKW-3微量呼吸仪对卵形鲳鲹(Trachinotus ovatus)胚胎和早期仔鱼的耗氧量进行测定,研究温度、盐度及pH的变化对胚胎耗氧量和Cu²⁺、Cd²⁺等重金属离子对早期仔鱼的耗氧量的影响。结果表明:在(25±0.5)℃条件下,卵形鲳鲹胚胎和早期仔鱼的耗氧量均随着发育时间的延长总体上呈上升的趋势,胚胎耗氧量在出膜前达到最大值,其中以原肠期和出膜期耗氧量变化最为显著。胚胎耗氧量随着温度、盐度、pH的增大而逐渐升高,在水温25℃、盐度35和pH为8时耗氧量到达最大值,随后逐渐减小。随着Cu²⁺浓度的升高,早期仔鱼耗氧量呈先增大后减小,在0.01mg·L^-1时耗氧率最大:胚胎耗氧量随着Cd²⁺浓度的升高而逐渐降低。

Study on oxygen consumption of embryo and early larva Trachinotus ovatus

Using SKW-3 instrument,we determined the oxygen consumption in main development stages of Trachinotus ovatus embryos and early larvae,and investigated the influences of temperature,salinity,pH on oxygen consumption of embryos,as well as effects of copper and cadmium concentrations on oxygen consumption of early larvae.The results showed that under the conditions of(25±0.5)℃, the oxygen consumption of embryos and early larvae of T.ovatus increased gradually with prolonged development,and became the maximum when embryos were in pre-stage of hatching.The stages of gastrula and hatching were sensitive especially to dissolved oxygen content in water.Embryonic oxygen consumption increased gradually with the increase of temperature,salinity and pH,reaching the maximum value at water temperature of 25℃,salinity 35 and pH 8,and then declined gradually.The oxygen consumption showed a trend of hyperbola with the rise of copper concentration.The oxygen consumption of embryos became the highest when copper concentration was 0.01 mg·L^-1 in water.Embryonic oxygen consumption decreased with the rise of cadmium concentration.