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| 基于rDNA ITS序列探讨粉煤灰污染对萼花臂尾轮虫种群遗传多样性的影响 | |
| 引用本文： | 张根,席贻龙,薛颖昊,胡忻,项贤领,温新利.基于rDNA ITS序列探讨粉煤灰污染对萼花臂尾轮虫种群遗传多样性的影响[J].生物多样性,2010,18(3):241-344. |
| 作者姓名： | 张根 席贻龙 薛颖昊 胡忻 项贤领 温新利 |
| 作者单位： | 1 安徽师范大学生命科学学院; 安徽省高校生物环境与生态安全省级重点实验室, 安徽芜湖 241000 2 南京大学现代分析测试中心, 江苏南京 210093 |
| 基金项目： | 国家自然科学基金，安徽省优秀青年基金滚动资助项目，安徽省重要生物资源的保护和利用研究重点实验窒专项基金资助项目，安徽省教育厅自然科学基金，安徽省高等学校优秀青年基金 |
| 摘 要： | 本文通过rDNA ITS序列测定和分析, 比较研究了粉煤灰污染水体(灰湖)与未污染水体(凤鸣湖和汀棠湖)萼花臂尾轮虫(Brachionus calyciflorus)的种群遗传多样性。结果表明, 灰湖萼花臂尾轮虫仅有1个姐妹种, 而两对照湖泊均存在2个姐妹种, 粉煤灰污染使萼花臂尾轮虫种复合体内姐妹种数量减少。就三个湖泊共有的姐妹种I而言, 灰湖轮虫种群单倍型多样性最低(h= 0.9516), 核苷酸多样性(π = 0.0066)低于汀棠湖(π = 0.0073)却高于凤鸣湖种群(π = 0.0052), 粉煤灰污染对萼花臂尾轮虫种群遗传多样性表现出一定的破坏作用; 凤鸣湖轮虫种群核苷酸多样性最低, 可能是由于水体中晶囊轮虫(Asplanchna)和桡足类动物捕食压力较高以及周围农田污染的联合作用造成的。三湖泊间, 仅汀棠湖与凤鸣湖轮虫种群间基因交流值较低(Nm = 1.95), 种群分化指数较高(Fst = 0.11358); 而灰湖与汀棠湖以及灰湖与凤鸣湖轮虫种群间基因交流水平较高(Nm均大于4), 种群分化指数较低(Fst分别为0.03535和0.00276)。AMOVA分析显示汀棠湖与凤鸣湖轮虫种群间变异所占比例较高, 为12.87%; 而灰湖与汀棠湖以及灰湖与凤鸣湖轮虫种群间变异所占比例较低, 分别为3.78%和2.78%。粉煤灰污染水体与未污染水体萼花臂尾轮虫种群间保持较高水平的基因交流, 种群分化不明显。 |
| 关 键 词： | 序列分析 电厂 遗传分化 重金属 水质分析 |
| 收稿时间： | 2010-02-02 |
Effects of coal ash pollution on the genetic diversity of Brachionus calyciflorus based on rDNA ITS sequences |
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| Gen Zhang,Yilong Xi,Yinghao Xue,Xin Hu,Xianling Xiang,Xinli Wen.Effects of coal ash pollution on the genetic diversity of Brachionus calyciflorus based on rDNA ITS sequences[J].Biodiversity Science,2010,18(3):241-344. | |
| Authors: | Gen Zhang Yilong Xi Yinghao Xue Xin Hu Xianling Xiang Xinli Wen |
| Institution: | 1 Provincial Key Laboratory of Biotic Environment & Ecological Safety in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000 2 Center of Modern Analysis, Nanjing University, Nanjing 210093 |
| Abstract: | In this study, rDNA ITS sequences were analyzed to compare the genetic diversity of Brachionus calyciflorus from the coal ash contaminated (Lake Hui) and two uncontaminated lakes (Lake Tingtang and Lake Fengming). The results showed that two sibling species in Brachionus calyciflorus species complex were defined in both Lake Tingtang and Lake Fengming, but only one sibling species was found in Lake Hui. The coal ash pollution decreased the number of sibling species. Based on the sequences of sibling species... |
| Keywords: | sequence analysis power plant genetic differentiation heavy metal water quality analysis |
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