| 野生稻Oryza nivara和O. rufipogon DNA甲基化多样性 |
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| 引用本文： | 崔影影,张大明.野生稻Oryza nivara和O. rufipogon DNA甲基化多样性[J].生物多样性,2010,18(3):227-450. |
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| 作者姓名： | 崔影影 张大明 |
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| 作者单位： | 中国科学院植物研究所系统与进化植物学国家重点实验室, 北京 100093 |
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| 摘 要： | 本文调查研究了野生稻群体内及群体间的DNA甲基化多样性。选取与亚洲栽培稻近缘的两个野生种Oryza nivara和O. rufipogon作为研究对象, 采用改进的MSAP (methylation-sensitive amplification polymorphism)技术对其基因组CCGG位点的甲基化多样性进行了分析。结果表明: 在同一个IRGC(the International Rice Germplasm Center)编号群体内的不同个体间, 基因组甲基化条带高度一致; 而在不同编号群体间, 甲基化条带表现为多态。其中后者又可以分为两类: 条带模式高度一致的Class I和条带模式呈多态性的Class II。将上述两类甲基化片段的编码基因与栽培稻粳稻(O. sativaL. subsp. japonica)和籼稻(O. sativa L. subsp. indica)两个亚种的同源基因进行序列比对发现, 在进化趋势上Class I表现得比较保守, 而Class II较为活跃。DNA甲基化多样性作为标志遗传多样性的一种信息来源, 其在群体分化及物种进化过程中的作用还需要进一步探讨。
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| 关 键 词： | 表观等位基因 MSAP 群体分化 进化 |
| 收稿时间： | 2009-10-19 |
Surveying DNA methylation diversity in the wild rice,Oryza nivara and O.rufipogon |
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| Yingying Cui,Daming Zhang.Surveying DNA methylation diversity in the wild rice,Oryza nivara and O.rufipogon[J].Biodiversity Science,2010,18(3):227-450. |
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| Authors: | Yingying Cui Daming Zhang |
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| Institution: | State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093 |
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| Abstract: | Intra-and inter-population methylation diversity at CCGG sites were surveyed in the genomes of Oryza nivara and O. rufipogon using a modified MSAP (methylation-sensitive amplification polymorphism) technique. These two wild species were closely related to cultivated rice in that they share the common A genome. Results revealed that methylated bands were uniform among different individuals of the same IRGC (International Rice Germplasm Center) accession, but polymorphic among different populations within the... |
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| Keywords: | epialleles MSAP population differentiation evolution |
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