Structural studies of the O-antigen of the lipopolysaccharide from an avirulent strain (M4S) of Pseudomonas solanacearum |
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| Authors: | Y Akiyama S Eda K Kato H Tanaka |
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| Institution: | Central Research Institute, The Japan Tobacco and Salt Public Corporation, 6-2 Umegaoka, Midori-ku, Yokohama 227 Japan;The Utsunomiya Tobacco Experiment Station, The Japan Tobacco and Salt Public Corporation, Oyama, Tochigi 323 Japan |
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| Abstract: | The structure of the O-antigen of the lipopolysaccharide from an avirulent strain (M4S) of Pseudomonas solanacearum has been investigated by methylation analysis, n.m.r. spectroscopy, and N-deacetylation-deamination, followed by analysis and controlled Smith-degradation of the product. These studies demonstrate that the O-antigen is composed of a tetrasaccharide repeating-unit having the following structure: ----3)-alpha-D-GlcpNAc-(1----2)-alpha-L-Rhap-(1----2)-alpha- L-Rhap-(1----3)- alpha-L-Rhap-(1----. |
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