13C-NMR and spectrophotometric studies of alcohol-lipid interactions |
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| Authors: | L L Herold E S Rowe R G Khalifah |
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| Affiliation: | 1. Department of Chemical Engineering, College of Technology, Can-Tho University Campus II, 3/2 street, Ninh Kieu district, Can Tho city, Viet Nam;2. Universite de Pau et des Pays de l''Adour, E2S UPPA, CNRS, TOTALENERGIES, LFCR, UMR5150, Pau, France;3. School of Mechanical & Mechatronics Engineering, Kyungsung University, 309, Suyeong-ro (Daeyeon-dong), Nam-gu, Busan 48434, Korea;4. Institute of Fundamental and Applied Sciences, Duy Tan University, Tran Nhat Duat Street, District 1, Ho Chi Minh City, Vietnam;5. Faculty of Environmental and Natural Sciences, Duy Tan University, 03 Quang Trung Street, Da Nang, Vietnam;1. Amity Institute of Biotechnology, Amity University Haryana, 122413, India;2. Solid State Physics Division, Bhabha Atomic Research Centre, Mumbai 400085, India;3. School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India;1. ESIQIE-SEPI, Departamento de Ingeniería Química, Instituto Politécnico Nacional, Ciudad de México 07738, Mexico;2. Institut für Technische Thermodynamik, Universität Stuttgart, Stuttgart 70569, Germany |
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| Abstract: | The interactions of butanol and mixtures of butanol and ethanol with dipalmitoylphosphatidyl choline (DPPC) liposomes have been investigated by both spectrophotometric measurements and Fourier transform 13C nuclear magnetic resonance spectroscopy. The spectrophotometric experiments indicate that butanol exhibits the same effects on the thermotropic properties of DPPC as the other short chain alcohols, methanol, ethanol and propanol, which have been shown to be characteristic of the alcohol induced transition of the lipid to the interdigitated state. An additive effect of butanol and ethanol on the induction of the interdigitated phase in DPPC was also observed. A decrease in line width and increase in T1 of the choline methyl signal were observed in the 13C-NMR experiments conducted at 32 degrees C when butanol was added to DPPC in increasing amounts suggesting an increase of disorder in the head group region of the lipid. Addition of ethanol to the NMR sample containing butanol produced hysteresis in the heating and cooling curves characteristic of the interdigitated state. In the interdigitated state, the choline methyl signal exhibited a T1 value equal to that when the lipid is in the fluid state. The increase of mobility in the head group region in the interdigitated gel state relative to the bilayer gel can be rationalized by the increase in surface area in that site when the lipid interdigitates. |
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