| 一株产低温碱性脂肪酶菌株的发酵条件优化 |
| |
| 引用本文： | 陈锋菊. 一株产低温碱性脂肪酶菌株的发酵条件优化[J]. 激光生物学报, 2010, 19(4): 516-520. DOI: 10.3969/j.issn.1007-7146.2010.04.019 |
| |
| 作者姓名： | 陈锋菊 |
| |
| 作者单位： | 湖南第一师范学院小教大专部,湖南,长沙,410002 |
| |
| 基金项目： | 湖南省教育厅基金项目 |
| |
| 摘 要： | 对一株产低温碱性脂肪酶细菌（Pseudoalteromonas sp．BJ17）的发酵条件进行了优化，研究各种碳源及氮源对产酶的影响，应用正交实验优化其发酵培养基组成。结果表明：最佳培养基组成为淀粉12g／L，蛋白胨12g／L，酵母膏3g／L，酪蛋白2g/L。最佳培养温度为25℃，发酵时间为16h。
|
| 关 键 词： | 低温脂肪酶 酶学性质 发酵条件 优化 |
Optimization of Fermentation Conditions for a Low-Temperature Lipase-Producing Strain |
| |
| CHEN Feng-ju. Optimization of Fermentation Conditions for a Low-Temperature Lipase-Producing Strain[J]. ACTA Laser Biology Sinica, 2010, 19(4): 516-520. DOI: 10.3969/j.issn.1007-7146.2010.04.019 |
| |
| Authors: | CHEN Feng-ju |
| |
| Affiliation: | CHEN Feng-ju (Primary Education Technical College Department, Hunan Firsh Normal college, Changsha 410002, Hunan, China) |
| |
| Abstract: | The culture of Pseudoalteromonas sp. B J17 were optimized for the production of alkaline protease. The optimum medium （ per liter） was consisted of 12 g starch, 12 g peptone, 3 g yeast extract, 2 g casein. The optimum fermentation conditions were determined as follows： temperature 25 ~C, and cultivation time 16 h. |
| |
| Keywords: | cold-adapted lipase enzymatic property fermentation condition optimization |
| 本文献已被 维普 万方数据 等数据库收录！ |
