| 离子交换色谱在细菌素分离纯化中的应用 |
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| 引用本文： | 陆泉,施波,李瑞胜,蔡庆霞,陈晓琳,张明. 离子交换色谱在细菌素分离纯化中的应用[J]. 中国微生态学杂志, 2010, 22(6): 570-572 |
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| 作者姓名： | 陆泉 施波 李瑞胜 蔡庆霞 陈晓琳 张明 |
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| 作者单位： | 安徽农业大学生命科学学院,安徽,合肥,230036 |
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| 基金项目： | 国家863计划项目，安徽省高校"十一五"优秀人才计划 |
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| 摘 要： | 近年来,乳酸菌细菌素在食品防腐剂和医药领域有着广泛的应用前景,而细菌素的分离纯化是其分子结构及遗传学特性等相关研究的重要基础。离子交换色谱是细菌素分离纯化的主要手段之一。本文阐述了离子交换色谱原理,分析了影响离子交换色谱分离纯化细菌素的各种因素,探讨了细菌素分离纯化中离子交换色谱条件的选择。
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| 关 键 词： | 离子交换色谱 细菌素 分离纯化 |
The application of ion exchange chromatography for the purification of bacteriocins |
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| LU Quan,SHI Bo,LI Rui-sheng,CAI Qing-xia,CHEN Xiao-lin,ZHANG Ming. The application of ion exchange chromatography for the purification of bacteriocins[J]. Chinese Journal of Microecology, 2010, 22(6): 570-572 |
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| Authors: | LU Quan SHI Bo LI Rui-sheng CAI Qing-xia CHEN Xiao-lin ZHANG Ming |
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| Affiliation: | (College of Life Science,Anhui Agriculture University,Hefei 230036,China) |
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| Abstract: | During the last few decades,lactic acid bacteria bacteriocins have been widely explored and investigated as food preservatives and pharmaceuticals. And the homogeneity bacteriocins are necessary for the studies of biochemical and genetic characterization. Ion Exchange Chromatography （IEC） is probably the most frequently used chromatographic technique for the purification of bacteriocins. In this paper,the principles of IEC,the effects of the various factors on the ion exchange process,and the experimental design of IEC for the purification of bacteriocins were reviewed. |
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| Keywords: | IEC Bacteriocin Purification |
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