Isolation and characterization of UDP-glycose: Cyanidin 3-O-glucosyltransferase from the flower buds ofSenecio x hybridus |
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| Authors: | Jun Ogata Susumu Teramoto Kunijiro Yoshitama |
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| Affiliation: | (1) Department of Biological Science, Faculty of Science, Kumamoto University, Kurokami 2-39-2, 860-8555 Kumamoto, Japan |
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| Abstract: | An UDPG: cyanidin 3-O-glucosyltransferase was isolated and purified about 260-fold from the flower buds ofSenecio x hybridus. The enzyme showed a pH optimum of 7.5 and no additional cofactors were required. The Km values for cyanidin and UDPG were 0.33 and 0.20 mM, respectively. Its molecular mass estimated by Sephacryl S-200 chromatography was 52 kDa. |
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| Keywords: | Compositae Flower buds Glucosyl-transferase Polyacylated anthocyanin Senecio× hybridus |
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