The inhibitory chloride channel of the lobster Panulirus penicillatus neuromuscular junction. |
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Authors: | E Gilat S W Jaslove |
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Affiliation: | Otto Loewi Center for Cellular and Molecular Neurobiology, Hebrew University of Jerusalem, Israel. |
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Abstract: | 1. Single channel activity was recorded from muscle membranes of the lobster Panulirus penicillatus using the patch-clamp technique. 2. Cell-attached, outside-out and inside-out patches were prepared from the deep abdominal extensor muscle. 3. Low amplitude single channel currents were observed in most patches, and were identified as being chloride-currents. 4. The chloride channel was active spontaneously, and tended to desensitize when outside-out patches were exposed to a small jet of glutamate. 5. Amplitude histograms of single channel currents presented a well defined peak of 8 pA at a membrane potential of -160 mV, while open and closed time histograms were fit to single exponential functions with tau open of 3.27 msec and tau closed of 31.58 msec. |
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