Preparative scale separation of neutral lipids and phospholipids by centrifugally accelerated thin-layer chromatography |
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| Authors: | S Bergheim K E Malterud T Anthonsen |
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| Affiliation: | Institute of Pharmacy, University of Oslo, Norway. |
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| Abstract: | Mixtures of lipids and phospholipids were separated by centrifugally accelerated thin-layer chromatography on a preparative scale (300-500 mg lipid mixture per run). The isolated lipids and phospholipids were identified by 1H and 13C NMR spectroscopy and their fatty acid composition was determined by GLC and GLC-MS of their methyl esters. |
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