Influence of gibberellic acid on auxin biosynthesis and their effects on coleoptile elongation in garlic |
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Authors: | S J Sata S J Gokani V S Thaker |
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Institution: | (1) Department of Biosciences, Saurashtra University, 360 005 Rajkot, India |
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Abstract: | To demonstrate the effect of auxin on intact coleoptile growth, garlic (Allium sativum L.) cloves were inoculated in agar supplemented with DW (control), GA3 and GA3+tryptophan (a precursor of IAA, GA3+T). The coleoptiles were harvested at 24 h intervals to measure growth in terms of length, activities of IAAld DH (which
convert tryptophan to IAA) and peroxidase (that oxidizes IAA). Contents of endogenous IAA and PAA were also measured by indirect
ELISA. Peroxidase activity was suppressed by GA3 treatment and increased by GA3+T treatment. Although endogenous contents of IAA were increased by the addition of GA3 and even more by GA3+T in the media, there was no further increase in coleoptile length, suggesting that garlic coleoptiles are sufficient in
their production of IAA. |
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Keywords: | Garlic (Allium sativum L ) Coleoptile Cytoplasmic peroxidase IAA IAAld DH PAA Wall bound peroxidase |
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