Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells |
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Authors: | Sarah Paule Katie Meehan Adam Rainczuk Andrew N Stephens Guiying Nie |
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Institution: | Prince Henrys Institute of Medical Research, Clayton 3168 Australia. sarah.paule@princehenrys.org. |
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Abstract: | Background Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation. Results Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics. Conclusions This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance. |
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