| 利用RAPD标记分析东亚地区桔梗的亲缘关系 |
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| 引用本文： | 严一字,吴基日. 利用RAPD标记分析东亚地区桔梗的亲缘关系[J]. 植物研究, 2007, 27(3): 308-312 |
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| 作者姓名： | 严一字 吴基日 |
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| 作者单位： | 东北林业大学，延边大学农学院 哈尔滨150040，延边大学农学院，龙井133400，龙井133400 |
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| 基金项目： | 吉林省科技发展计划;吉林省科技厅科研项目 |
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| 摘 要： | 对取自中国、日本、韩国和朝鲜等东亚地区的24个桔梗种质资源，利用RAPD-PCR标记方法，分析了其遗传变异和遗传关系，旨在为桔梗的品种鉴定和杂交育种的亲本选择提供理论依据。结果表明： （1）用11个引物扩增出131条谱带，平均每个引物扩增出11.9条谱带，并扩增出61个多态性谱带，平均每个引物扩增出5.5条多态性谱带，显示出了相对高的多态率（46.6%）。（2）得到的RAPD数据的遗传相似性范围为0.668～0.994，并以遗传相似系数0.78为标准，将24个桔梗种质资源分为7个类群。
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| 关 键 词： | 桔梗；RAPD；亲缘关系 |
| 修稿时间： | 2006-09-12 |
Genetic Relationships of Platycodon grandiflorum Cultivars from East-Asian Area Using RAPD Markers |
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| YAN Yi-Zi,WU Ji-Ri. Genetic Relationships of Platycodon grandiflorum Cultivars from East-Asian Area Using RAPD Markers[J]. Bulletin of Botanical Research, 2007, 27(3): 308-312 |
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| Authors: | YAN Yi-Zi WU Ji-Ri |
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| Affiliation: | 1. Northeast Forestry University, Harbin 150040;2. Agricultural College of Yanbian University, Longjing 133400 |
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| Abstract: | The genetic variation and genetic relationships among 24 samples of platycodon grandiflorum DC.cultivars collected from East-Asian area(China,Japan,Korea and DPRK)were analyzed by the method of RAPD-PCR markers.The results showed that 131 bands were amplified by 11 primers with an average of 11.9 bands per primer and 61 polymorphic bands were obtained with an average of 5.5 bands per primer,which revealed relatively high percentage of polymorphic bands(46.6%).The genetic similarity calculated from RAPD data varied from 0.668 to 0.994,and the 24 samples of Platycodon grandiflorum DC.cultivars were divided into 7 major groups on a criterion of 0.78 coefficient. |
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| Keywords: | RAPD |
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