| 中药蓼大青叶及其伪品的nrDNA ITS区序列的测定 |
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| 引用本文： | 李国强,王峥涛,李晓波,徐国钧.中药蓼大青叶及其伪品的nrDNA ITS区序列的测定[J].中国野生植物资源,2001,20(3):43-46. |
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| 作者姓名： | 李国强 王峥涛 李晓波 徐国钧 |
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| 作者单位： | 中国药科大学生药学研究室,南京,210038 |
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| 摘 要： | 中药蓼大青叶的基源为蓼科蓼属植物蓼蓝（Polygonum tinctorium Ait)，同属植物的水蓼（辣蓼），（P.hydropiperL.)的叶常作其伪品，本文采用PCR直接测序法，首次测定了它们的核糖体DNA ITS区序列，结果显示，两者充列长度（ITS1，5，8S，ITS2）均为646bp，序列间共有28个变异位点，本研究为产蓝植物的DNA分子鉴定提供了必要的序列资料。
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| 关 键 词： | 蓼科 蓼属 蓼蓝 水蓼 核糖体DNA ITS区序列 中草药 大青叶 伪品 |
Determination of nrDNA ITS Sequence of LIAODAQINGYE (FOLIUM POLYGONI TINCTORH)and its Counterfeit |
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| Li Guoqiang,Wang Zhengtao,Li Xiaobo,Xu Guojun.Determination of nrDNA ITS Sequence of LIAODAQINGYE (FOLIUM POLYGONI TINCTORH)and its Counterfeit[J].Chinese Wild Plant Resources,2001,20(3):43-46. |
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| Authors: | Li Guoqiang Wang Zhengtao Li Xiaobo Xu Guojun |
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| Abstract: | As the traditional Chinese Medicine ,Liaodaqingye (Folium Polygoni Tinctorii) is from Polygonum tinotorium Ait.Its counterfeit form P. hydropiper L. Their nuclear ribosomal DNA internal transcribed spacer (ITS)regions were first sequenced. Sequence analysis showed that the ITS region(including 5.8S coding reion)of P.tinctorium Ait. is 646bp in length, and that of P.hydropiper L.is 646bp.There are 28 variable sites (including 5.8 S coding region) in pairwise comparison of the sequence.This study could provide significant data for molecular identification of indigo plant at DNA level. |
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| Keywords: | Polygonaceae L Polygonum L Polygonrm tinctorium Ait Polygonum hydropiper L nrDNA ITS sequence |
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