螺旋藻对实验性急性肝损伤保护作用机制初探

目的初步探讨螺旋藻对实验性急性肝损伤的保护作用机制。方法实验大鼠随机分为4组：空白组（KB）,生理盐水组（NS）,甘利欣组（GLX）和螺旋藻组（LXZ）。分组口服灌胃5 d,用四氯化碳（CC l4）建立急性大鼠肝损伤模型后继续灌胃2 d后取材,检测肝功、抗氧化指标、肠道菌群定量、内毒素、肿瘤坏死因子和病理。结果螺旋藻具有保肝作用。与甘利欣组比较,能明显保护肠道微生态平衡（P〈0.05）,内毒素和肿瘤坏死因子升高程度不如甘利欣组明显（P〈0.05）,SOD（超氧化物歧化酶）和px-GSH（还原型谷胱甘肽转移酶）水平明显偏高（P〈0.05）。结论提示螺旋藻不仅通过抗氧化起到抗肝损伤作用,而且还可通过调节肠道菌群紊乱—内毒素—肿瘤坏死因子α途径起到护肝效果。

Protective mechanism of Spirulina on experimental acute liver injury

Objective To explore the effects of Spinalina on endotoxin,TNF-α, SOD, MDA and pxGHS in rat acute hepatic injury. Methods Rat model was conducted using Wistar rats. The rats randomized into four groups ： KB, NS, GANLIXIN（GLX） and spirulina. The quantitative analysis of intestinal flora was conducted. Endotoxin and TNF-α were determined by immunology. SOD,MDA and pxGHS were also analysed. Results Spirulina had effects to reducing ALT,AST, and could also module intestinal flora. Compared with the model groups, contents of endotoxin and TNF-α were obviously lower in spinalina group than those of GLX group （ P 〈 0.05 ）. Spirulina could significantly strengthened SOD, pxGSH activity and reduced contend of MDA. Conclusion The results indicated that Spirulina has effects to protecting hepatic function by the pathways of prebiotic-endotoxin-TNF-α and antioxidation.