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A genetic linkage map of Theobroma cacao L.
Authors:C Lanaud  A M Risterucci  A K J N'Goran  D Clement  M H Flament  V Laurent  M Falque
Institution:(1) CIRAD-Biotrop, BP 5035, F-34032 Montpellier Cedex, France;(2) IDEFOR/DCC and CIRAD-CP, 01 BP, 1827, 01 Abidjan, Ivory Coast
Abstract:A linkage map of the cocoa genome comprising 193 loci has been constructed. These loci consist of 5 isozymes, 101 cDNA/RFLPs, 4 loci from genes of known function, 55 genomic DNA/RFLPs and 28 RAPDs. A population of 100 individuals derived from a cross between two heterozygous genotypes was used. Segregation analyses were performed with the JoinMap program. Ten linkage groups, which putatively correspond to the ten gametic chromosomes of cocoa, were identified. The map covers a total length of 759 cM with a 3.9 cM average distance between 2 markers. A small fraction (9%) of the markers deviated significantly from the expected Mendelian ratios.
Keywords:Theobroma cacao  Isozyme  RFLP RAPD  Linkage map
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