| 丝裂霉素C对瘢痕疙瘩成纤维细胞Cyclin D1、CDK4及P27基因表达的影响 |
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| 引用本文： | 刘莺,于冬梅,郝立君,肖志波,李颖,任丽虹,罗赛.丝裂霉素C对瘢痕疙瘩成纤维细胞Cyclin D1、CDK4及P27基因表达的影响[J].中国组织化学与细胞化学杂志,2008,17(2):126-131. |
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| 作者姓名： | 刘莺 于冬梅 郝立君 肖志波 李颖 任丽虹 罗赛 |
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| 作者单位： | 哈尔滨医科大学附属第二医院整形美容科,哈尔滨,150086 |
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| 摘 要： | 目的探讨丝裂霉素C（Mitomycin C，MMC）对瘢痕疙瘩成纤维中Cyclin D1、CDK4及P27基因表达的影响，以阐明MMC对瘢痕疙瘩的作用机制，为瘢痕疙瘩的治疗提供理论依据。方法用不同浓度的MMC作用于体外培养的瘢痕疙瘩成纤维细胞，MTT法测量细胞增殖情况，利用半定量RT-PCR、免疫组化分析用药前后Cydin D1、CDK4及P27的mRNA和蛋白水平表达的变化。结果MMC可明显抑制体外培养的瘢痕疙瘩成纤维细胞的增殖，并呈时间和剂量依赖性抑制成纤维细胞增殖；MMC作用后，使Cyclin D1和CDK4的mRNA及蛋白表达减少，P27基因的表达增加。结论MMC可能是通过调节cyclin D1／CDK4／P27细胞周期调控通路，抑制瘢痕疙瘩成纤维细胞增殖而发挥治疗瘢痕疙瘩的作用。
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| 关 键 词： | 丝裂霉素C 瘢痕疙瘩成纤维细胞 CyclinD1 CDK4 P27 细胞周期 |
| 修稿时间： | 2007年8月8日 |
INFLUENCE OF MITOMYCIN C ON THE EXPRESSIONS OF CYCLIN D1,CDK4 AND P27 GENE OF KELOID FIBROBLASTS |
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| Liu Ying,Yu Dongmei,Hao Lijun,Xiao Zhibo,Li Ying,Ren Lihong,Luo Sai.INFLUENCE OF MITOMYCIN C ON THE EXPRESSIONS OF CYCLIN D1,CDK4 AND P27 GENE OF KELOID FIBROBLASTS[J].Chinese Journal of Histochemistry and Cytochemistry,2008,17(2):126-131. |
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| Authors: | Liu Ying Yu Dongmei Hao Lijun Xiao Zhibo Li Ying Ren Lihong Luo Sai |
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| Institution: | Liu Ying,Yu Dongmei,Hao Lijun,Xiao Zhibo,Li Ying,Ren Lihong,Luo Sai(Department of Plastic Surgery,The Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China)= |
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| Abstract: | Objective To investigate the expression of cyclin D1,CDK4 and P27 in cultured fibroblasts derived from keloids before and after treatment with mitomycin C(MMC),and to elucidate the mechanism of MMCs inhibiting effects on keloids.Methods Cultured fibroblasts derived from keloids were subjected to MMC of different concentrations.The proliferation of fibroblasts was detected by MTT.The expression of cyclin D1,CDK4 and P27 was detected by RT-PCR and immunohistochemical method.Results MMC significantly inhibited... |
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| Keywords: | Mitomycin C Keloid fibroblast(KFB) Cyclin D1 CDK4 P27 Cell cycle |
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