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Mechanoformation of neutral giant phospholipid vesicles in high ionic strength solution |
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| Authors: | Pavlič Janez I Genova Julia Popkirov George Kralj-Iglič Veronika Iglič Aleš Mitov Marin D |
| Institution: | aUniversity of Ljubljana, Faculty of Electrical Engineering, Tr?aška 25, 1000 Ljubljana, Slovenia;bUniversity of Ljubljana, Faculty of Health Sciences, Zdravstvena pot 5, 1000 Ljubljana, Slovenia;cBulgarian Academy of Sciences, Institute of Solid State Physics, Tzarigradsko Chaussee Blvd. 72, 1784 Sofia, Bulgaria;dUniversity of Ljubljana, Faculty of Medicine, Lipi?eva 2, 1000 Ljubljana, Slovenia |
| Abstract: | We present new and simple method for formation of giant unilamellar vesicles (GUVs) in high ionic strength solutions, such as phosphate buffered saline (PBS). Mechanoformation method is an alternative method to electroformation method. The advantage of the mechanoformation procedure is that there are no limitations with respect to the ionic strength of the aqueous solutions, because there is no applied electric potential thus no current flow through the formation cell and no electrolysis is induced. |
| Keywords: | Mechanoformation Neutral phospholipid Giant phospholipid vesicle High ionic strength solution |
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