Plectin Rodless Isoform Expression and Its Detectiona in Mouse Brain |
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Authors: | Peter?Fuchs Daniel?Spazierer Email author" target="_blank">Gerhard?WicheEmail author |
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Institution: | (1) Department of Molecular Cell Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria;(2) Department of Molecular Cell Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9, A-1030, Vienna, Austria |
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Abstract: | Summary The widely expressed cytolinker protein plectin shows extensive isoform diversity both at the N-terminus and in the central
part of the molecule. Judged on mRNA data, plectin variants lacking the central rod domain are expressed at a ∼20-fold lower
level than full-length proteins and their detection on the protein level can be difficult. Here we present data on the expression
of plectin rodless isoforms in mouse brain and in rat glioma C6 cells on RNA and protein levels. Our data indicate that among
the rodless variants expressed in neuronal tissues, those starting with exon 1c (plectin 1c) seem to be the most prominent
ones. In addition, we show that similar to other monoclonal antibodies reported in the literature, the widely used mAb 7A8
recognizes an epitope within plectin's rod domain and therefore is unsuited to detect rodless variants of plectin. |
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Keywords: | cytoskeleton cytolinker plectin rodless isoform |
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