The reduced extracellular calcium requirement for proliferation by neoplastic hepatocytes |
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| Authors: | Swierenga S. H. H. Whitfield J. F. Morris H. P. |
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| Affiliation: | (1) Division of Biological Sciences, National Research Council of Canada, KIA 0R6, Ottawa, Canada;(2) Present address: Department of Biochemistry, Howard University School of Medicine, 20001 Washington, D.C. |
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| Abstract: | Summary Cells from neonatal rat livers were unable to maintain DNA-synthetic activity in calcium-deficient medium, but neoplastic hepatocytes from Morris hepatomas 5123 tc and 7795 synthesized DNA and proliferated indefinitely in this calcium-deficient medium. The calcium content of fresh hepatoma tissue from which these cultures were derived was as much as 10 times greater than that of normal liver; but this difference could not account for the insensitivity of neoplastic cells to extracellular calcium because it disappeared during subsequent cultivation in vitro. NRCC No. 16597 |
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| Keywords: | liver cell culture Morris hepatomas calcium proliferation DNA synthesis neoplastic transformation |
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