牛血清白蛋白与地肤提取物N-反式阿魏酰酪胺相互作用研究

该研究以一年生白及幼苗为材料，通过测定生物量、氨基酸含量、蛋白质及多糖含量的变化，研究不同浓度的芸苔素内酯（BR）、萘乙酸（NAA）和茉莉酸甲酯（Me JA）喷施对白及幼苗假鳞茎快速生长发育影响。结果表明：1.6 × 10 4 mmol·L 1的BR处理组，假鳞茎鲜重达3.39 g/株，高于其他处理组，分别是清水对照(CK1)和沼气肥对照组(CK2)的1.19倍和1.25倍；单个假鳞茎（单株）的新生萌芽数也高于其他处理，达到2.17个，说明生产效益至少可提高19%。把两个对照组和三种植物生长调节物质处理后假鳞茎产量最高的处理组（即1.6 ×10 4 mmol·L 1的BR、0.5 mmol·L 1的NAA和0.25 mmol·L 1的Me JA）进一步测定假鳞茎氨基酸、蛋白质和多糖含量，发现CK2氨基酸含量最高，高达8.58%，而CK1含量最低，仅为5.21%，三种植物生长调节物质处理组分别是7.26%、7.53%和5.69%。蛋白质含量由高到低依次是沼气肥对照组、1.6 ×10 4 mmol·L 1 BR、0.5 mmol·L 1 NAA、0.25 mmol·L 1 ME JA和清水对照组，它们的含量分别是11.6%、11.0%、10.5%、9.14%、7.72%，这说明与氨基酸含量基本一致。三种植物生长调节物质处理后白及多糖含量分别为24.2%、26.5%、26.5%，均远高于清水对照（19.3%）和沼气肥对照（21.8%）。综合分析认为，1.6 ×10 4 mmol·L 1的BR能同时提高白及假鳞茎的产量和质量。该研究结果对于白及规模化种植栽培具有重要指导意义。

Interaction between bovine serum albumin and N-trans-feruloyltyramine from Kochia scoparia

Kochia scoparia, also called broom seedlings, broom dishes and peacock pine, is an annual herb of genus Kockia in family chenopodiaceae. The seedlings of K. scoparia are wild vegetables with high protein, low fat and rich potassium and carotene. As a widely distributed plant in china, K. scoparia is eaten as traditional wild herbs. However, there is little research and development on its medicinal value. The N-trans-feruloyltyramine from K. scoparia was isolated and purified by silica gel column chromatography. The interavtion mechanism between N-trans-feruloyltyramine and bovine serum albumin(BSA)was investigated by UV and fluorescence spectrometry. The quenching constant, binding constant and binding sites were obtained by analyzing quenching data based on Stern-Volrner equation. The results showed that the fluorescence of BSA was quenched by N-trans-feruloyltyramine by a static quenching process strong interaction between N-trans-feruloyltyramine and BSA could be stored and transported by protein. The results of this experiment provide some theoretical information for the further development and medicinal value of Kochia scoparia resources.