高黎贡山中山湿性常绿阔叶林树种的分布格局及其地形影响因子

基因组甲基化修饰受环境因素的影响。在以甲基化为代表的表观遗传学研究中，如何减少保存环境对异地采后样品的影响，提高整个实验的准确性和科学性，目前尚未有系统的认知。该研究选取5种常用的采后样品保存方式（液氮冷冻、-20 ℃冷冻、变色硅胶干燥、密封袋密封、75%酒精浸泡），分别用Wilcoxon signed ranks tests统计分析和UPGMA聚类分析方法，对华南植物园锥栗进行F MSAP研究，以期找出最佳保存方式。同时，利用正交试验法对F MSAP体系进行优化，筛选出9对引物（E3 H/M2；E5 H/M2；E6 H/M1；E6 H/M5；E8 H/M1；E8 H/M5；E9 H/M2；E11 H/M5；E14 H/M1），并对不同发育时期的锥栗甲基化水平及遗传多样性进行了论述。结果表明：在锥栗F MSAP的研究中，Willcoxon signed ranks tests统计分析和UPGMA聚类分析结论一致，密封袋保存效果最佳；成熟叶半甲基化率（27.83%）和总甲基化率（51.13%）高于幼叶（21.35%，45.90%），全甲基化率（23.30%）低于幼叶（24.55%），平均多态位点百分数39.60%，香农信息指数0.207±0.002，表现出较高的甲基化水平和遗传多样性。

Distribution pattens of tree species and its influencing factors of topography in a mid-montane humid ever-green broad-leaved forest in Gaoligong Mountains

The change features for distribution pattern of tree species under different sampling areas and growth stages to explore the influence of topographic factors on the distribution pattern of tree species were analyzed. As the mid-montane humid ever-green broad-leaved forest in the Gaoligong Mountains National Nature Reserve of Yunnan Province was taken as the research object, such approaches as diffusion coefficient, variance analysis and multiple comparison were adopted, based on the topographic data and community survey data of 200 m × 200 m dynamic monitoring plots. The results showed that the majority trees in the community exhibited aggregated distributions and a few species were randomly distributed. The distribution pattern of most tree species had nothing to do with the sampling area, most of which exhibited aggregated distributions under each sampling area; the distribution pattern of a few tree species was affected by sampling area, and they were randomly distributed in small areas and exhibited aggregated distributions in large areas. The distribution pattern of about half of the trees in the community was independent of its growth stage, most of which exhibited aggregated distributions in all growth stages. On the other hand, the distribution pattern of the other half species was affected by the growth stage: most young trees exhibited aggregated distribution, and middle trees and large trees showed random distribution. The variance analysis demonstrates that terrain heterogeneity has an important effect on aggregated distribution of most tree species, and that the differentiation of tree species in resource niche is the controlling factor, while the terrain redistribution of hydrothermal resources is an important factor.