有关SARS病原冠状病毒合并感染组织胞浆菌的探讨

目的探讨SARS病原学的冠状病毒复合组织胞浆菌感染的问题。方法对象为SARS患者尸检1例和南猴(某猴场病死猴)尸检1例,采用病理组织学和免疫组化荧光检测的方法作检查,观察和比较两者尸检材料肺、脾、淋巴结和肝的病理变化和组织内组织胞浆菌的存在。结果1.人SARS和南猴的病理组织学改变极为相似。2.免疫组化结果表明,用人SARS恢复期血清和兔抗组织胞浆菌血清与组织胞浆菌抗原均呈阳性反应,显示此例SARS患者曾感染组织胞浆菌,其次用此两种抗血清分别与人SARS和南猴肺、脾、淋巴结尸检材料反应,均有组织胞浆菌阳性菌体的存在,说明人SARS和南猴脏器均有组织胞浆菌的感染。结论人SARS病原除冠状病毒外,还有组织胞浆菌的合并感染。

Exploration on SARS-Coronavirus Infection Combined with Histoplasma Capsulatum

Objective To explore the etiology of SARS that it could be coronavirus infection combined with Histoplasma Capsulatum. Methods Specimens taken from one case of SARS autopsy and one case of rhesus monkey necropsy. Histopathological and immunofluorescence techniques were used to detect the fungal Histoplasma Capsulatum and pathological changes in the specimens of human and monkey's lung, spleen, lymph nodes and liver. Results 1. The histopathological changes of monkey were very similar to those in human beings. 2. The results of immunohistochemistry demonstrated that: (a) SARS convalescent serum and rabbit anti-Histoplasma Capsulatum serum vs. H. Capsulatum antigen revealed to be positive. (b) Both two kinds of serum vs. specimens of SARS and monkey's lungs, spleen, lymph nodes and liver appeared H. Capsulatum positive. Conclusion It was evident that the patient and monkey were infected with H. Capsulatum in vivo. The etiology of SARS is coronavirus combined with Histoplasma Capsulatum infection.